Borrelia burgdorferi b31 DNA sequences in PCR products
of Alzheimer disease autopsy brain DNA extractions
of Alzheimer disease autopsy brain DNA extractions
22 nucleotide sequence from
Borrelia burgdorferi
Flagellin B ORF BBO 147
TGGGAGTTTCTGGTAAGATTAA
click here
Borrelia burgdorferi
Flagellin B ORF BBO 147
TGGGAGTTTCTGGTAAGATTAA
click here
14 nucleotide sequence
from Borrelia burgdorferi
Flagellin B ORF BBO 147
CTCAACAGCCAGCA
from Borrelia burgdorferi
Flagellin B ORF BBO 147
CTCAACAGCCAGCA
21 nucleotide sequence from
Borrelia burgdorferi Flagellin B
ORF BBO 147
GAAGGAGCTCAACAGCCAGCA
Borrelia burgdorferi Flagellin B
ORF BBO 147
GAAGGAGCTCAACAGCCAGCA
Features in this part of subject sequence:
flagellar filament 41 kDa core protein (flaB)
Score = 41.0 bits (44), Expect = 2e-04
Identities = 22/22 (100%), Gaps = 0/22 (0%)
Strand=Plus/Minus
Query 50 TGGGAGTTTCTGGTAAGATTAA 71
||||||||||||||||||||||
Sbjct 148523 TGGGAGTTTCTGGTAAGATTAA 148502
Features in this part of subject sequence:
flagellar filament 41 kDa core protein (flaB)
Score = 39.2 bits (42), Expect = 8e-04
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Minus
Query 319 GAAGGAGCTCAACAGCCAGCA 339
|||||||||||||||||||||
Sbjct 148008 GAAGGAGCTCAACAGCCAGCA
147988
flagellar filament 41 kDa core protein (flaB)
Score = 39.2 bits (42), Expect = 8e-04
Identities = 21/21 (100%), Gaps = 0/21 (0%)
Strand=Plus/Minus
Query 319 GAAGGAGCTCAACAGCCAGCA 339
|||||||||||||||||||||
Sbjct 148008 GAAGGAGCTCAACAGCCAGCA
147988
Human chromosome 11q
7 of 7 Transfections contain between 200 to 241 nucleotides from
chromosome 11q
The actual lengths of the 11q match region is dependent on the individual
lengths of the Transfection sequences;
Maximum Transfection length is 339 bases and this specimen matches
241 bases in the 11 q reference sequence
Shorter Transfection sequences with lengths of approximately 300 bases
demonstrate 100% matches
with the reference 11q sequences but are "truncated" to lengths
dependent on the total Transfection sequence length
7 of 7 Transfections contain between 200 to 241 nucleotides from
chromosome 11q
The actual lengths of the 11q match region is dependent on the individual
lengths of the Transfection sequences;
Maximum Transfection length is 339 bases and this specimen matches
241 bases in the 11 q reference sequence
Shorter Transfection sequences with lengths of approximately 300 bases
demonstrate 100% matches
with the reference 11q sequences but are "truncated" to lengths
dependent on the total Transfection sequence length
| **************************************************** |
BLASTSearch using tblastx menu choice for Transfection
DQ132793.1
Demonstrates a contribution of a Plasmid of Borrelia
burgdorferi , Bo126plasmid B0126_lp36 (Accession
CP001567.1) to the 5' end (approximately covering
positions 1-94) Click here to see sequence
DQ132793.1
Demonstrates a contribution of a Plasmid of Borrelia
burgdorferi , Bo126plasmid B0126_lp36 (Accession
CP001567.1) to the 5' end (approximately covering
positions 1-94) Click here to see sequence
Note: The Plasmids of Borrelia species
are subject to genetic changes over
time. Considering the hypothesis that
Borrelia infections in each of the 7
Alzheimer's patients occurred 30 years
or more before they died,it is plausible
to postulate that the nucleotide
sequences of the plasmids underwent
genetic changes which would result in
partial sequence change diversities as
compared with specimens currently on
file in the NCBI Database
are subject to genetic changes over
time. Considering the hypothesis that
Borrelia infections in each of the 7
Alzheimer's patients occurred 30 years
or more before they died,it is plausible
to postulate that the nucleotide
sequences of the plasmids underwent
genetic changes which would result in
partial sequence change diversities as
compared with specimens currently on
file in the NCBI Database
| Reference: Review: The plasmids of Borrelia burgdorferi: essential genetic elements of a pathogen Philip E. Stewart , Rebecca Byram , Dorothee Grimm, Kit Tilly, Patricia A. Rosa Plasmid, 2005,13:1-13 |
| Reference: Infection and Immunity,2000,68:1574-86 (Diversities in the Plasmid families cp32/18 of Borrelia burgdorferi) |
| Additional Data for the nucleotides 1-96 in the DQ132793.1 Transfection Product |
Reference:
Molecular Microbiology
2005, 58:625-635
(Genomic plasticity and
mechanisms for gene
reshuffling in Borrelia
burgdorferi)
Molecular Microbiology
2005, 58:625-635
(Genomic plasticity and
mechanisms for gene
reshuffling in Borrelia
burgdorferi)
| Diagram: From Stewart et al, Plasmid, 2005,53:1-13 |
