Borrelia burgdorferi DNA is present in Autopsy brain tissue- A comparison of two
published studies (References below)
Present Study
Harvard Alzheimer Autopsy
Brain slices (10 cases)
Species: Homo sapiens
PCR primers to detect a
portion of Borrelia burgdorferi
B31 DNA (Flagellin B)
Yale University School of Medicine
Primate Autopsy brain tissues
Species: Rhesus monkeys (  6 cases)
PCR primers to detect multiple
portions of Borrelia burgdorferi DNA
Open Reading Frames (ORFs)
(Including Flagellin B)
Borrelia burgdorferi exposure
of subjects in the study
Unknown
Borrelia burgdorferi exposure of
subjects in the study
Cutaneous inoculation in the laboratory
Time Interval between Borrelia
infection and autopsy-
Unknown
Time Interval between Borrelia
infection and autopsy-
13 weeks-71 weeks
Design of Primers for
detection of Flagellin B DNA of
Borrelia burgdorferi -
Primer Premiere Software with
Complete DNA sequence of
Borrelia burgdorferi obtained
from www.tigr.com
Design of primers for detection
of Flagellin B DNA of Borrelia
burgdorferi -
Complete DNA sequence OF
Borrelia burgdorferi B31
PCR - Number of primers used
to detect Flagellin B DNA-

Two pairs of primers (4
primers)
Negative Control material
One Autopsy human  brain
without clinical or pathological
evidence of Alzheimer's
disease
Negative Control material -
No normal uninfected primates examined-
The experimental design was to measure
up regulation and down regulation of Open
Reading Frames (ORF) of Borrelia in
infected immuncompetent primates
versus
Borrelia genome expression in infected
steroid treated animals
PCR technique -

DECAL cDNA
RT PCR
PCR technique

Multiplex
PCR - Number of primers used
to detect Flagellin B DNA-
( 2 primers) per ORF examined
Gel Electrophoresis of PCR
products -
One sharp band at site
predicted for the PCR product
Gel Electrophoresis of PCR
products -
One sharp band at site
predicted for the PCR product
DNA sequence analysis of
each PCR product -
Completed for 7 cases
DNA sequence analysis of each
PCR product-
NOT PERFORMED
Detection of a portion of the
DNA of Borrelia burgdorferi
Flagellin B
Positive results in 7 of 10
Alzheimer disease brains
Detection of a portion of the DNA
of Borrelia burgdorferi Flagellin B

Positive results in all infected
primate brains
Conclusions based on
experimental data:
Portions of Flagellin B DNA of
Borrelia burgdorferi are
present in some cases of
autopsy Alzheimer's disease
brain tissue
Conclusions based on experimental
data:
DNA transcriptomes for Flagellin B
DNA of Borrelia burgdorferi are
present, but are either upregulated or
downregulated, in  autopsy brain
tissues of Immuncompetent versus
steroid treated cases of primates who
have been infected with a cutaneous
injection in the laboratory.
Reference:
Transfection "Junk" DNA - a link to the
pathogenesis of Alzheimer's disease?,
Med Hypotheses. 2006;66(6):1140-1.
Epub 2006 Feb 14


www.ncbi.nlm.nih.gov/pubmed/16481123
Reference:
Borrelia burgdorferi transcriptome in
the central nervous system of
non-human primates, 2003,
Proceedings of the National Academy
of Sciences, 100: 15953-15958

www.pnas.org/cgi/doi/10.1073/pnas.24
32412100
Data from Figure 3, p 15957,
PNAS,2003, 100, 15953-15958.
Narasimhan, S. ...Fikrig, E. et al
DNA gel electrophoresis of Alzheimer
brain DNA digests; Multiplex PCR using
Primers for BBO 147 ORF of Borrelia
burgdorferi
Click here to see
primers