| Borrelia burgdorferi DNA is present in Autopsy brain tissue- A comparison of two published studies (References below) |
| Present Study Harvard Alzheimer Autopsy Brain slices (10 cases) Species: Homo sapiens PCR primers to detect a portion of Borrelia burgdorferi B31 DNA (Flagellin B) |
Yale University School of Medicine
Primate Autopsy brain tissues
Species: Rhesus monkeys ( 6 cases)
PCR primers to detect multiple
portions of Borrelia burgdorferi DNA
Open Reading Frames (ORFs)
(Including Flagellin B)
Primate Autopsy brain tissues
Species: Rhesus monkeys ( 6 cases)
PCR primers to detect multiple
portions of Borrelia burgdorferi DNA
Open Reading Frames (ORFs)
(Including Flagellin B)
| Borrelia burgdorferi exposure of subjects in the study Unknown |
Borrelia burgdorferi exposure of
subjects in the study
Cutaneous inoculation in the laboratory
subjects in the study
Cutaneous inoculation in the laboratory
| Time Interval between Borrelia infection and autopsy- Unknown |
Time Interval between Borrelia
infection and autopsy-
13 weeks-71 weeks
infection and autopsy-
13 weeks-71 weeks
| Design of Primers for detection of Flagellin B DNA of Borrelia burgdorferi - Primer Premiere Software with Complete DNA sequence of Borrelia burgdorferi obtained from www.tigr.com |
Design of primers for detection
of Flagellin B DNA of Borrelia
burgdorferi -
Complete DNA sequence OF
Borrelia burgdorferi B31
of Flagellin B DNA of Borrelia
burgdorferi -
Complete DNA sequence OF
Borrelia burgdorferi B31
| PCR - Number of primers used to detect Flagellin B DNA- Two pairs of primers (4 primers) |
| Negative Control material One Autopsy human brain without clinical or pathological evidence of Alzheimer's disease |
Negative Control material -
No normal uninfected primates examined-
The experimental design was to measure
up regulation and down regulation of Open
Reading Frames (ORF) of Borrelia in
infected immuncompetent primates
versus
Borrelia genome expression in infected
steroid treated animals
No normal uninfected primates examined-
The experimental design was to measure
up regulation and down regulation of Open
Reading Frames (ORF) of Borrelia in
infected immuncompetent primates
versus
Borrelia genome expression in infected
steroid treated animals
PCR technique -
DECAL cDNA
RT PCR
DECAL cDNA
RT PCR
| PCR technique Multiplex |
PCR - Number of primers used
to detect Flagellin B DNA-
( 2 primers) per ORF examined
to detect Flagellin B DNA-
( 2 primers) per ORF examined
| Gel Electrophoresis of PCR products - One sharp band at site predicted for the PCR product |
Gel Electrophoresis of PCR
products -
One sharp band at site
predicted for the PCR product
products -
One sharp band at site
predicted for the PCR product
| DNA sequence analysis of each PCR product - Completed for 7 cases |
DNA sequence analysis of each
PCR product-
NOT PERFORMED
PCR product-
NOT PERFORMED
| Detection of a portion of the DNA of Borrelia burgdorferi Flagellin B Positive results in 7 of 10 Alzheimer disease brains |
Detection of a portion of the DNA
of Borrelia burgdorferi Flagellin B
Positive results in all infected
primate brains
of Borrelia burgdorferi Flagellin B
Positive results in all infected
primate brains
| Conclusions based on experimental data: Portions of Flagellin B DNA of Borrelia burgdorferi are present in some cases of autopsy Alzheimer's disease brain tissue |
Conclusions based on experimental
data:
DNA transcriptomes for Flagellin B
DNA of Borrelia burgdorferi are
present, but are either upregulated or
downregulated, in autopsy brain
tissues of Immuncompetent versus
steroid treated cases of primates who
have been infected with a cutaneous
injection in the laboratory.
data:
DNA transcriptomes for Flagellin B
DNA of Borrelia burgdorferi are
present, but are either upregulated or
downregulated, in autopsy brain
tissues of Immuncompetent versus
steroid treated cases of primates who
have been infected with a cutaneous
injection in the laboratory.
Reference:
Transfection "Junk" DNA - a link to the
pathogenesis of Alzheimer's disease?,
Med Hypotheses. 2006;66(6):1140-1.
Epub 2006 Feb 14
www.ncbi.nlm.nih.gov/pubmed/16481123
Transfection "Junk" DNA - a link to the
pathogenesis of Alzheimer's disease?,
Med Hypotheses. 2006;66(6):1140-1.
Epub 2006 Feb 14
www.ncbi.nlm.nih.gov/pubmed/16481123
Reference:
Borrelia burgdorferi transcriptome in
the central nervous system of
non-human primates, 2003,
Proceedings of the National Academy
of Sciences, 100: 15953-15958
www.pnas.org/cgi/doi/10.1073/pnas.2432412100
Borrelia burgdorferi transcriptome in
the central nervous system of
non-human primates, 2003,
Proceedings of the National Academy
of Sciences, 100: 15953-15958
www.pnas.org/cgi/doi/10.1073/pnas.2432412100
Data from Figure 3, p 15957,
PNAS,2003, 100, 15953-15958.
Narasimhan, S. ...Fikrig, E. et al
PNAS,2003, 100, 15953-15958.
Narasimhan, S. ...Fikrig, E. et al
DNA gel electrophoresis of Alzheimer
brain DNA digests; Multiplex PCR using
Primers for BBO 147 ORF of Borrelia
burgdorferi
brain DNA digests; Multiplex PCR using
Primers for BBO 147 ORF of Borrelia
burgdorferi
